Enhancing LC/ESI-MS/MS Throughput for Plasma Bile Acid Assay by Derivatization-based Sample-Multiplexing
نویسندگان
چکیده
منابع مشابه
An Improved GC Method for Rapid Analysis of Valproic Acid in Human Plasma Without Derivatization
A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®<span styl...
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After the completion of the Human Genome Project has provided a blueprint of the DNA present in each human cell, genomics research is now focusing on the study of DNA variations that occur between individuals, seeking to understand how these variations confer susceptibility to common diseases such as diabetes or cancer. The most common form of genomic variation are the so called single nucleoti...
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Targeted mass spectrometry assays for protein quantitation monitor peptide surrogates, which are easily multiplexed to target many peptides in a single assay. However, these assays have generally not taken advantage of sample multiplexing, which allows up to ten analyses to occur in parallel. We present a two-dimensional multiplexing workflow that utilizes synthetic peptides for each protein to...
متن کاملan improved gc method for rapid analysis of valproic acid in human plasma without derivatization
a simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (vpa) in human plasma has been developed. total time for sample preparation and gc analysis is less than 45 min. after plasma protein precipitation, vpa was extracted into chloroform with suitable recovery. by using stabilwax®-da capillary gc column, a symmetrical gas chromatographic peak was ...
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BACKGROUND Plasma Citrulline concentration has been correlated to functional enterocyte mass. Dried blood spot (DBS) analysis using LC-MSMS reduces sample amount needed. We optimized DBS elution to increase precision and accuracy of DBS LC-MSMS analysis. METHOD DBS control samples were eluted in varying pH (2.2-7.0) and for varying times (15-75 min) and Cit, Arg and Orn were analyzed using LC...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2020
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.20p082